Chirazyme
Chloroperoxidase
The Only Suppliers of Enzyme Chloroperoxidase
About Chloroperoxidase
In enzymology, a chloride peroxidase (EC 1.11.1.10) is an enzyme that catalyzes the chemical reaction 2 RH + 2 Cl- + H2O2 \rightleftharpoons 2 RCl + 2 H2OThe 3 substrates of this enzyme are RH, Cl−, and H2O2, whereas its two products are RCl and H2O.This enzyme belongs to the family of oxidoreductases, specifically those acting on a peroxide as acceptor (peroxidases). The systematic name of this enzyme class is chloride: hydrogen-peroxide oxidoreductase. This enzyme is also called chloroperoxidase. It employs one cofactor which may be either heme or vanadia.Chloroperoxidases are heme or vanadium-containing enzymes that exhibits peroxidase, catalase and cyto-chrome P450-like activities in addition to catalyzing halogenation reactions[1]. Despite functional similarities with other heme enzymes, CPO folds into a novel tertiary structure dominated by eight helical segments. The catalytic base, required to cleave the peroxide O-O bond, is glutamic acid rather than histidine as in other peroxidases.
The History of Chirazyme
Lowell P. Hager, Founder 1926-2014
Professor Lowell P. Hager, who passed away peacefully on April 15, 2014 at the age of 87, was an internationally recognized enzymologist and protein chemist best known for his fundamental studies on halogenating enzymes and heme peroxidases that are widespread throughout biology. Professor Hager founded Chirazyme Labs and also served as the Director of the Biotechnology Center and the Cell and Molecular Biology Training Program. Among his many awards and honors, Professor Hager was elected to the National Academy of Sciences in 1995, and was the inaugural recipient of the William Rutter Chair in Biochemistry in 1996.
Products & Pricing
Chloroperoxidase from Caldariomyces fumago
Commercial Grade
EC 1.11.1.10
CAS 9055-20-3
Rz (A400/A280) = 1-1.2
Activity – 20-40,000 U/ml, One international unit of enzyme catalyzes the formation of 1 umole per minute of dichlorodimedone at 25 degrees C.Description – 2-5% (wt/v) Aqueous solution in 0.1 M phosphate pH 4.5Stability – Stable at 4 degrees C. Freezing is not recommended as it results in approximately 50% loss of activity.Orders of up to 100,000 Units $5 per 1,000 Units (minimum order of $100)Orders of 100,000 -1,000,000 Units $3.75 per 1,000 Units
Research Grade
EC 1.11.1.10
CAS 9055-20-3
Rz (A400/A280) = 1.2-1.4Activity – 20-40,000 U/ml, One international unit of enzyme catalyzes the formation of 1 umole per minute of dichlorodimedone at 25 degrees C.Description – 2-5% (wt/v) Aqueous solution in 0.1 M phosphate pH 4.5Stability – Stable at 4 degrees C. Freezing is not recommended as it results in approximately 50% loss of activity.Research grade CPO is HPLC purified on an as needed basisOrders $8 per 1,000 Units (minimum order of $100).
Please inquire about pricing and lead times for orders in excess of 1 MU.
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